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control aav vector  (Addgene inc)


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    Structured Review

    Addgene inc control aav vector
    Control Aav Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pampk+alpha2+delta312x/pAMPK+alpha2+delta312X+(Plasmid+%2360127)/pm36435289-57-27-30
    Average 91 stars, based on 4 article reviews
    control aav vector - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Generated:

    Article Title: The hexosamine biosynthesis pathway is a targetable liability in KRAS/LKB1-mutant lung cancer
    Article Snippet: H460- and H2122-EV, -LKB1 WT were generated by infecting cells with pBABE retroviral vectors expressing no cDNA or cDNAs encoding wild type LKB1. pBABE-FLAG-LKB1 WT were from Lewis Cantley (Addgene plasmid #8592). .. Calu1- and H1373-shGFP, shLKB1 were generated by infecting cells with pLentiX2-PURO-shLKB1-Hu and pLentiX2 control. pLentiX2-PURO-shLKB1-Hu was from R. Shaw (Addgene 61242) and pAMPK alpha2 delta312X (constitutively active AMPK) was from M. Birnbaum (Addgene plasmid 60127). ..

    Article Title: CPS1 maintains pyrimidine pools and DNA synthesis in KRAS/LKB1-mutant lung cancer cells
    Article Snippet: All cell lines have been DNA fingerprinted for provenance using the PowerPlex 1.2 kit (Promega) and were mycoplasma free using the e-Myco kit (Boca Scientific). .. H460-EV, -LKB1 WT, and -LKB1 KD were generated by infecting cells with pBABE retroviral vectors expressing no cDNA or cDNAs encoding wild type or kinase-dead (K78I mutant) LKB1, respectively. pBABE-FLAG-LKB1 WT and KD were from Lewis Cantley (Addgene plasmid #8592 and #8593, respectively) and pAMPK alpha2 delta312X (constitutively active AMPK) was from Morris Birnbaum (Addgene plasmid #60127). .. For murine CPS1 (mCPS1) cloning, mCPS1 cDNA was purchased from GE healthcare (cloneID: 40098767), and subcloned into pWPXL lentiviral plasmid (Addgene, plasmid #12257).

    Control:

    Article Title: The hexosamine biosynthesis pathway is a targetable liability in KRAS/LKB1-mutant lung cancer
    Article Snippet: H460- and H2122-EV, -LKB1 WT were generated by infecting cells with pBABE retroviral vectors expressing no cDNA or cDNAs encoding wild type LKB1. pBABE-FLAG-LKB1 WT were from Lewis Cantley (Addgene plasmid #8592). .. Calu1- and H1373-shGFP, shLKB1 were generated by infecting cells with pLentiX2-PURO-shLKB1-Hu and pLentiX2 control. pLentiX2-PURO-shLKB1-Hu was from R. Shaw (Addgene 61242) and pAMPK alpha2 delta312X (constitutively active AMPK) was from M. Birnbaum (Addgene plasmid 60127). ..

    Plasmid Preparation:

    Article Title: The hexosamine biosynthesis pathway is a targetable liability in KRAS/LKB1-mutant lung cancer
    Article Snippet: H460- and H2122-EV, -LKB1 WT were generated by infecting cells with pBABE retroviral vectors expressing no cDNA or cDNAs encoding wild type LKB1. pBABE-FLAG-LKB1 WT were from Lewis Cantley (Addgene plasmid #8592). .. Calu1- and H1373-shGFP, shLKB1 were generated by infecting cells with pLentiX2-PURO-shLKB1-Hu and pLentiX2 control. pLentiX2-PURO-shLKB1-Hu was from R. Shaw (Addgene 61242) and pAMPK alpha2 delta312X (constitutively active AMPK) was from M. Birnbaum (Addgene plasmid 60127). ..

    Article Title: CPS1 maintains pyrimidine pools and DNA synthesis in KRAS/LKB1-mutant lung cancer cells
    Article Snippet: All cell lines have been DNA fingerprinted for provenance using the PowerPlex 1.2 kit (Promega) and were mycoplasma free using the e-Myco kit (Boca Scientific). .. H460-EV, -LKB1 WT, and -LKB1 KD were generated by infecting cells with pBABE retroviral vectors expressing no cDNA or cDNAs encoding wild type or kinase-dead (K78I mutant) LKB1, respectively. pBABE-FLAG-LKB1 WT and KD were from Lewis Cantley (Addgene plasmid #8592 and #8593, respectively) and pAMPK alpha2 delta312X (constitutively active AMPK) was from Morris Birnbaum (Addgene plasmid #60127). .. For murine CPS1 (mCPS1) cloning, mCPS1 cDNA was purchased from GE healthcare (cloneID: 40098767), and subcloned into pWPXL lentiviral plasmid (Addgene, plasmid #12257).

    Retroviral:

    Article Title: CPS1 maintains pyrimidine pools and DNA synthesis in KRAS/LKB1-mutant lung cancer cells
    Article Snippet: All cell lines have been DNA fingerprinted for provenance using the PowerPlex 1.2 kit (Promega) and were mycoplasma free using the e-Myco kit (Boca Scientific). .. H460-EV, -LKB1 WT, and -LKB1 KD were generated by infecting cells with pBABE retroviral vectors expressing no cDNA or cDNAs encoding wild type or kinase-dead (K78I mutant) LKB1, respectively. pBABE-FLAG-LKB1 WT and KD were from Lewis Cantley (Addgene plasmid #8592 and #8593, respectively) and pAMPK alpha2 delta312X (constitutively active AMPK) was from Morris Birnbaum (Addgene plasmid #60127). .. For murine CPS1 (mCPS1) cloning, mCPS1 cDNA was purchased from GE healthcare (cloneID: 40098767), and subcloned into pWPXL lentiviral plasmid (Addgene, plasmid #12257).

    Expressing:

    Article Title: CPS1 maintains pyrimidine pools and DNA synthesis in KRAS/LKB1-mutant lung cancer cells
    Article Snippet: All cell lines have been DNA fingerprinted for provenance using the PowerPlex 1.2 kit (Promega) and were mycoplasma free using the e-Myco kit (Boca Scientific). .. H460-EV, -LKB1 WT, and -LKB1 KD were generated by infecting cells with pBABE retroviral vectors expressing no cDNA or cDNAs encoding wild type or kinase-dead (K78I mutant) LKB1, respectively. pBABE-FLAG-LKB1 WT and KD were from Lewis Cantley (Addgene plasmid #8592 and #8593, respectively) and pAMPK alpha2 delta312X (constitutively active AMPK) was from Morris Birnbaum (Addgene plasmid #60127). .. For murine CPS1 (mCPS1) cloning, mCPS1 cDNA was purchased from GE healthcare (cloneID: 40098767), and subcloned into pWPXL lentiviral plasmid (Addgene, plasmid #12257).

    Mutagenesis:

    Article Title: CPS1 maintains pyrimidine pools and DNA synthesis in KRAS/LKB1-mutant lung cancer cells
    Article Snippet: All cell lines have been DNA fingerprinted for provenance using the PowerPlex 1.2 kit (Promega) and were mycoplasma free using the e-Myco kit (Boca Scientific). .. H460-EV, -LKB1 WT, and -LKB1 KD were generated by infecting cells with pBABE retroviral vectors expressing no cDNA or cDNAs encoding wild type or kinase-dead (K78I mutant) LKB1, respectively. pBABE-FLAG-LKB1 WT and KD were from Lewis Cantley (Addgene plasmid #8592 and #8593, respectively) and pAMPK alpha2 delta312X (constitutively active AMPK) was from Morris Birnbaum (Addgene plasmid #60127). .. For murine CPS1 (mCPS1) cloning, mCPS1 cDNA was purchased from GE healthcare (cloneID: 40098767), and subcloned into pWPXL lentiviral plasmid (Addgene, plasmid #12257).



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